Difference between CDS and cDNA - Biology Stack Exchange
Nov 8, 2013 · What is the difference between Coding Sequences (CDS) and cDNA? Are Coding sequences the sequences that is transcribed to mRNA and cDNA in contrast DNA obtained by …
Minimum amount of RNA for cDNA conversion and qPCR
Nov 28, 2019 · I searched several protocols for reverse transcription of RNA and all suggest using RNA concentrations of 1ug. Could a lower amount result in inferior transcript representation …
Why do we use cRNA instead of initial extracted RNA in the …
I am recapping for the exam in transcriptomics and stumbled into a question about microarray. So, our regular workflow is to extract RNA from cells generate cDNA from the initial RNA using …
Storing Taqman plates with polymerase in fridge before running
May 10, 2015 · @MarchHo the cDNA has been prepared ahead of time and frozen. On the day of the qPCR, I prepare the reaction using 2 dye-labeled probes (gene of interest and internal …
Regarding the difference between cDNA library and RNA …
Aug 22, 2014 · The construction of a cDNA library involves making a DNA 'copy' of the mRNA because the aim is to synthesise a large set of recombinant plasmid molecules, each …
Why is the start of my coding sequence ATG and not TAC?
Mar 3, 2021 · Which cDNA to convert it to, the template or the non-template? From @NoahSprent's answer, I now know that the template is the opposite from the strand with the …
Why use DNA polymerase in making cDNA? - Biology Stack …
Nov 17, 2013 · RT is capable of synthesizing a complementary dna strand ( as in HIV life cycle.) Then why is DNA pol used when cDNA (synthesizing the second strand of it ) has to be …
Complementarity of cDNA - Biology Stack Exchange
Strictly speaking, what is the definition of cDNA? This confuses me, since usually it is said to refer to DNA that is complementary to mRNA. Is this correct? Is it restricted to mature mRNA? I ...
lab techniques - Why is cDNA usually lacking in terminal …
May 29, 2022 · Traditionally cDNA cloning used an oligo (dT) primer that binds to the 3′ poly (A) tail of a mRNA and extends towards the 5′ end of the mRNA. What makes it difficult for the …
Clarification of the procedures to amplify cDNA needed
Dec 3, 2013 · The typical procedure to obtain cDNA from genomic extracted DNA is the following: RNA is extracted from desired tissue, RNA/DNA hybrid is obtained by virtue of reverse …