Full-length RecE and RecT from Rac prophage mediate highly efficient linear–linear homologous recombination that can be used to clone large DNA regions directly from genomic DNA into expression ...

Young scientists often get frustrated with laborious experimental procedures, such as cloning, PCR, or sequencing. However, the Internet has made planning and performing these procedures faster and ...

Molecular cloning refers to the replication and recombination of DNA molecules. The first cloning experiments were carried out in the 1970s, when restriction endonucleases were discovered. Restriction ...

The availability of effective vector systems is a pre-requisite for genetic manipulation of plants through recombinant DNA technology. We report here construction of a series of binary vectors that ...

Lentiviruses have the unique ability amongst retroviruses of being able to infect non-cycling cells. Vectors derived from lentiviruses have provided a huge advancement in technology and seemingly ...

Nereus Gunther is a molecular biologist at the Agricultural Research Service within the US Department of Agriculture (Washington D.C., USA), where part of his research focuses on Campylobacter and its ...